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Section on Genetics of Communication Disorders
Dennis Drayna, Ph.D., Chief
In the Section on Genetics of Communication Disorders (Laboratory of Communication Disorders), we use family- and population-based genetic methods to identify genes responsible for human communication disorders. The lab has a major focus on the speech disorder stuttering, for which we have identified causative mutations in several genes. We study the biochemical and cellular effects of these mutations, and we use mouse model systems to study the effects of these mutations on mouse ultrasonic vocalizations. The long-term goal of our work is to identify specific neuronal pathologies caused by these mutations.
We also perform studies on the sense of taste, where our overall goal is understanding how naturally occurring genetic variation contributes to differences in the sense of taste in humans. We are currently focused on a study of variation in taste perception genes and tobacco use, with a particular interest in menthol tobacco use and variation in the TRPM8 gene, which encodes the menthol receptor.
Stuttering: We study families in which many individuals stutter. Genetic linkage studies can identify the location of the gene or genes responsible for stuttering in these families. We have enrolled a large group of families in Pakistan, where marriages between cousins are common. Each of these families has multiple cases of stuttering, and our genetic linkage studies identified major linkage signals, indicating different causative genes, on chromosomes 12, 3, and 16.
We have also identified a large, English-speaking family in Cameroon, West Africa, in which stuttering occurs in more than 40 family members. Linkage studies in this family have shown that stuttering is due to several different genes, each acting in a different branch of the family. These genes are located on chromosome 2, 3, 14, and 15. Recent studies of stuttering families from Brazil have identified yet another locus, on chromosome 10, that carries a stuttering gene in this population.
Studies of the chromosome 12 locus have identified causative mutations in the gene GNPTAB, and additional studies have identified causative mutations in the related GNPTG and NAGPA genes. Together these three genes appear to account for up to 15 percent of stuttering cases in the U.S., as well as elsewhere in the world. Studies of the chromosome 15 locus in the Cameroonian family have recently identified mutations in the AP4E1 gene, which encodes part of a complex involved in trafficking components within cells. Additional studies demonstrated that the product of the NAGPA gene interacts with the AP4 complex, and thus all of the stuttering genes identified to date reveal deficits in the mechanism cells use to move components to their proper location within the cell. Mutations in AP4E1 appear to account for up to 5 percent of stuttering cases in worldwide populations.
We have engineered mouse lines that carry mutations that cause stuttering in humans. Like the humans who carry these mutations, these mice are normal in all ways measured to date, except that they display subtle differences in their ultrasonic vocalizations. We are using these mouse lines to attempt to identify the cells within the brain that mediate this abnormal vocalization.
Taste perception: Our current studies are focused on taste perception and tobacco use, particularly flavored tobacco use. We are studying variants in genes encoding components of the human taste perception machinery, including genes encoding taste receptors, to evaluate the hypothesis that such genetic variation is associated with mentholated tobacco use, or with tobacco use in general. Menthol tobacco use displays wide divergence among different ethnic groups in the United States, and we seek to determine whether the strong ethnic differences could be accounted for by genetically encoded differences in taste perception.
- Dennis Drayna, Ph.D. Senior Investigator +1 301 402 4930 (Send e-mail (link sends e-mail))
- Carlos Eduardo Frigerio Domingues, Ph.D. Postdoc Fellow (Visiting Fellow) +1 301 594 1043 (Send e-mail (link sends e-mail))
- Tae-Un Han Post-Doc Visititing Fellow +1 301 443 0278 (Send e-mail (link sends e-mail))
- Eduardo Sainz Chemist +1 301 402 4232 (Send e-mail (link sends e-mail))
- Frigerio-Domingues C, Drayna D. Genetic contributions to stuttering: The current evidence. Mol Genet Genomic Med. 2017 Feb 19;5(2):95-102. eCollection 2017 Mar.
- Barnes TD, Wozniak DF, Gutierrez J, Han TU, Drayna D, Holy TE. A mutation associated with stuttering alters mouse pup ultrasonic vocalizations. Curr Biol. 2016 Apr 13. pii: S0960-9822(16)30179-8.
- Brewer CC, Zalewski CK, King KA, Zobay O, Riley A, Ferguson MA, Bird JE, McCabe MM, Hood LJ, Drayna D, Griffith AJ, Morell RJ, Friedman TB, Moore DR. Heritability of non-speech auditory processing skills. Eur J Hum Genet. 2016 Aug;24(8):1137-44. Epub 2016 Feb 17.
- Raza MH, Mattera R, Morell R, Sainz E, Rahn R, Gutierrez J, Paris E, Root J, Solomon B, Brewer C, Basra MAR, Khan S, Riazuddin S, Braun A, Bonifacino J, Drayna D. Association between rare variants in AP4E1, a component of intracellular trafficking, and persistent stuttering. American Journal of Human Genetics 2015; 97:715-725.
- Raza M, Domingues C, Webster R, Sainz E, Paris E, Rahn, R, Gutierrez J, Chow HM, Mundorff J, Kang C, Riaz N, Basra M, Khan S, Riazuddin S, Moretti-Ferreira D, Braun A, and Drayna D. Mucolipidosis Type II/III alpha and non-syndromic stuttering are associated with different variants in the same genes. European Journal of Human Genetics advance online publication 1 July 2015; doi: 10.1038/ejhg. 2015.154.
- Han T-U, Park J, Domingues C, Moretti-Ferreira D, Paris E, Sainz E, Gutierrez J., and Drayna D. A study of the role of the FOXP2 and CNTNAP2 genes in persistent developmental stuttering. Neurobiology of Disease 2014; 69:23-31.
- Domingues CE, Olivera CMC, Oliveira BV, Juste FS, Andrade CF, Giacheti CM, Moretti-Fereira D, Drayna D. A genetic linkage study in Brazil identifies a new locus for persistent developmental stuttering on chromosome 10. Genetics and Molecular Research 2014; 13:2094-2101.
- Raza MH, Gertz EM, Mundorff J, Lukong J, Kuster J, Schäffer A, Drayna D. Linkage analysis of a large African family segregating stuttering suggests polygenic inheritance and assortative mating. Human Genetics 2013; 132:385-396.
- Raza, MH, Ali RA, Riazuddin S, Drayna D. Studies in a consanguineous family reveal a novel locus for stuttering on chromosome 16q. Human Genetics 2012; 131:311-313.
- Lee W-S, Kang C, Drayna D, Kornfeld S. Analysis of Mannose 6-Phosphate uncovering enzyme mutations associated with persistent stuttering. Journal of Biological Chemistry 2011; 286:39786-39793.
- Raza MH, Riazuddin S, Drayna D. Identification of an autosomal recessive stuttering locus on chromosome 3q13.2-3q13.33. Human Genetics 2010; 128:461-463.
- Kang C, Riazuddin S, Mundorff J, Krasnewich D, Friedman P, Mullikin JC, Drayna D. Mutations in the lysosomal enzyme-targeting pathway and persistent stuttering. N Engl J Med. 2010 Feb 25;362(8):677-85. Epub 2010 Feb 10.
- Riaz N, Steinberg S, Ahmad J, Pluzhnikov A, Raizuddin S, Cox N, Drayna D. Genomewide significant linkage to stuttering on chromosome 12. American Journal of Human Genetics 2015; 76:647-651.
- Kozlitina J, Risso D, Lansu K, Olsen RHJ, Sainz E, Luiselli D, Barik A, Frigerio-Domingues C, Pagani L, Wooding S, Kirchner T, Niaura R, Roth B, Drayna D. An African-specific haplotype in MRGPRX4 is associated with menthol cigarette smoking. PLoS Genet 15(2): e1007916.
- Risso D, Sainz E, Morini G, Tofanelli S, Drayna D. Taste perception of Antidesma bunius fruit and its relationships to bitter taste receptor gene haplotypes. Chem Senses. 2018 Aug 24;43(7):463-468.
- Risso D, Behrens M, Sainz E, Meyerhof W, Drayna D. Probing the evolutionary history of human bitter taste receptor pseudogenes by restoring their function. Mol Biol Evol. 2017 Jul 1;34(7):1587-1595.
- Risso D, Sainz E, Gutierrez J, Kirchner T, Niaura R, Drayna D. Association of TAS2R38 haplotypes and menthol cigarette preference in an African American cohort. Nicotine Tob Res. 2017 Apr 1;19(4):493-494.
- Risso DS, Kozlitina J, Sainz E, Gutierrez J, Wooding S, Getachew B, Luiselli D, Berg CJ, Drayna D. Genetic variation in the TAS2R38 bitter taste receptor and smoking behaviors. PLoS One. 2016 Oct 6;11(10):e0164157.
- Risso DS, Mezzavilla M, Pagani L, Robino A, Morini G, Tofanelli S, Carrai M, Campa D, Barale R, Caradonna F, Gasparini P, Luiselli D, Wooding S, Drayna D. Corrigendum: Global diversity in the TAS2R38 bitter taste receptor: Revisiting a classic evolutionary PROPosal. Sci Rep. 2016 Jun 27;6:28406.
- Roudnitzky N, Risso D, Drayna D, Behrens M, Meyerhof W, Wooding SP. Copy number variation in TAS2R bitter taste receptor genes: Structure, origin, and population genetics. Chem Senses. 2016 Oct;41(8):649-59. Epub 2016 Jun 23.
- Risso DS, Mezzavilla M, Pagani L, Robino A, Morini G, Tofanelli S, Carrai M, Campa D, Barale R, Caradonna F, Gasparini P, Luiselli D, Wooding S, Drayna D. Global diversity in the TAS2R38 bitter taste receptor: Revisiting a classic evolutionary PROPosal. Sci Rep. 2016 May 3;6:25506. Erratum in: Sci Rep. 2016 Jun 27;6:28406.