This video demonstrates a method that allows semi-automated quantification of hair cells in the entire utricle by staining adult utricle cultures for Pou4f3, a hair cell-specific transcription factor. The provided utricle images and the ImageJ macro script have been used in the instructional video to demonstrate this method. Users can download these image files and use them to familiarize themselves with this ImageJ macro-based counting method.
Video Credit: NIDCD Section on Sensory Cell Biology
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