The larger form of myosin 15 (green) is found in
the shorter rows of mouse hair cell stereocilia
(magenta). New evidence indicates that this larger
version of myosin 15 is involved in maintaining the
shorter stereocilia, which play a key role in detecting
sound. Credit: Jonathan E. Bird and Thomas B.
A research team led by scientists at the National Institute on Deafness and Other Communication Disorders (NIDCD), part of the National Institutes of Health (NIH), has discovered that a protein essential for building key hearing structures in the inner ear also plays a critical role in maintaining them throughout life. The researchers report that healthy hearing involves two distinct forms of a molecular motor protein called myosin 15 (MYO15A)—one form that helps build stereocilia, and a second, much longer, version of the protein that is needed to maintain stereocilia. Stereocilia are the finger-like projections that extend from the surface of hair cells, the inner ear's sensory cells.
The discovery could lay the foundation for new approaches to preserve hearing and prevent or minimize the most common forms of hearing loss. Approximately 15 percent of Americans (26 million people) between the ages of 20 and 69 have high frequency hearing loss due to exposure to noise at work or during leisure activities.
The study, appearing October 6 in the journal eLife, was a collaboration between the NIDCD, the University of Michigan Medical School Department of Human Genetics, and the University of Kentucky. Jonathan Bird, Ph.D., is the communicating author and a postdoctoral fellow in the NIDCD Laboratory of Molecular Genetics. The study is additionally highlighted in eLife by an accompanying "Insight" article.
The new work builds upon decades of research by NIDCD scientists and others to understand the formation and function of stereocilia. Stereocilia are like antennae, sensing sound waves passing through the ear and converting them to electrical signals that are transmitted to the brain. They are arranged in rows of increasing length on the hair cell surface, forming a staircase-like structure that is critical to their function.
Researchers have previously shown that some mutations in myosin 15 stunt stereocilia growth and prevent them from reaching their normal height, causing profound deafness. Scientists believe that myosin 15, which like other motor proteins can move around the cell, helps to build stereocilia by delivering critical component parts.
The current study was prompted by an earlier discovery of an unusual, deafness-associated mutation in the myosin 15 gene in a large Pakistani family. Previously studied myosin 15 mutations lie in the latter half of the gene, but this mutation lies toward the front of the gene. The affected family members did not have as substantial a hearing loss as expected based on work with the earlier mutations. This difference led the scientists to wonder if there were two forms of myosin 15, each with a separate function in the inner ear.
The structure of the myosin 15 gene also suggested to the researchers that it could produce two forms of the protein—a full-length form and a shorter form, lacking the front end. The novel mutation would only affect the longer version, leaving the shorter one intact. This could explain why the associated deafness was slightly less severe than for the previously studied mutations, which would damage both forms.
To further explore the possibility that there are two versions of myosin 15 involved in hearing, the researchers altered the gene in mice to have the same mutation as the unusual one found in the Pakistani family. Like their human counterparts, these mice produced the shorter form but lacked the longer one. By comparing them to normal mice and to mice missing both forms, the researchers would be able to distinguish any unique functions associated with each version.
When they examined the mice with the altered gene, they found that early on, these mice could hear loud sounds in their most sensitive frequency range, but by 4 to 6 weeks, their hearing had quickly progressed to profound deafness. These findings mirrored the researchers' observation that the mice's hair cells initially produced typical staircase-shaped arrays of stereocilia, but that over time, progressively lost the shorter stereocilia "steps" at the bottom.
"We were shocked when we first looked at the young mice and saw that the stereocilia appeared normal," said Dr. Bird. "The other mouse models, which lack both versions of myosin 15, produce stunted stereocilia from the beginning, so we knew right away that something completely different was going on here."
The results revealed that while the shorter version of myosin 15 is sufficient for the construction of the stereocilia staircase, the longer version plays a critical role in maintaining it. Imaging studies showed that the shorter form is produced predominantly early in development throughout the staircase, while the longer form is largely made later and localizes to the shorter rows, providing further support for their distinct roles.
Together, the findings show that myosin 15, through its dual forms, not only helps construct the stereocilia staircase of hair cells, but also works to maintain them, a process that until now has been poorly understood.
"We know that noise-induced damage to stereocilia is a major contributor to age-related hearing loss," said Thomas B. Friedman, Ph.D., chief of the NIDCD Laboratory of Molecular Genetics. "By digging deeper and learning more about the mechanisms used by myosin 15 to maintain stereocilia, we may be able to find ways to enhance the process and possibly preserve hearing to some extent in the later years."
This work was supported by funds from the NIDCD intramural research program (DC000039–18 and DC000048–18), NIDCD extramural funds (R01–DC005053, R01–DC008861, P30–DC005188), the Hearing Health Foundation, a University of Michigan Barbour Scholarship, and a James V. Neel Fellowship.