Advanced Imaging Core
Ronald S. (Ron) Petralia, Ph.D., Staff Scientist
Building 50, Room 4142
50 South Drive, Bethesda, MD 20892-8027
Phone: (301) 496-3804
Fax: (301) 480-0126, E-mail: email@example.com
Dr. Petralia received a B.S. from University of Massachusetts Amherst in 1975, and a Ph.D. from Texas A&M University in 1979, and has been a practicing electron microscopist for 35 years. He practiced light and electron microscopic immunocytochemistry at the NIDCD, in the laboratory of Dr. Robert J. Wenthold (deceased) from 1991-2009, prior to establishing the Advanced Imaging Core.
Ya-Xian Wang, M.D., Biologist
Dr. Wang earned an M.D. from Harbin Medical University, P.R. China in 1977, then was Doctor-in-Charge in Ophthalmology/Eye Surgery in Heilongjiang Provincial Hospital in Harbin until she came to the United States. She worked in the laboratory of Dr. Robert J. Wenthold from 1993-2009 on electron microscopy immunocytochemistry and primary neuron cell culture.
The Advanced Imaging Core (AIC) offers investigators specialized centralized core laboratory support services. The primary goal of the AIC is to support the research interests and ongoing projects of various NIDCD laboratories within the Division of Intramural Research (DIR). The AIC provides the resources necessary to assist in the proper performance of a variety of specialized experiments and in the interpretation of obtained data. The AIC provides specific expertise, new technologies, and resources to enhance the research efforts of all NIDCD investigators. The team specializes in transmission electron microscope (TEM) studies of immunocytochemistry and ultrastructure, but also does confocal and other light microscope (LM) analyses for correlative LM/EM studies.
Cell biological studies, including physiology, genetics, immunocytochemistry, etc., always consider what happens at the molecular level. Transmission electron microscopy allows scientists to come closer than ever to studying cell composition at the molecular level. The AIC’s TEM specialists offer training and related assistance to principal investigators at the NIDCD, and contribute to collaborative studies with scientists at the NIDCD, NIH, and beyond.
Tissue preparation with freeze-substitution
NIDCD research papers published since 2010
- Szarama, K.B., N. Gavara, R.S. Petralia, M.W. Kelley, and R.S. Chadwick (2012) Cytoskeletal changes in actin and microtubules underlie the developing surface mechanical properties of sensory and supporting cells in the mouse cochlea. Development (in press).
- Seabold, .G.K., P.Y. Wang, R.S. Petralia, K. Chang, A. Zhou, M.I. McDermott, Y.-X. Wang, S.L. Milgram, and R.J. Wenthold (2012) Dileucine and PDZ binding motifs mediate synaptic adhesion-like molecule 1 (SALM1) trafficking in hippocampal neurons. J. Biol. Chem. 287:4470-4484.
- Ren, D., H. Xie, W. Zhang, F. Hassan, R.S. Petralia, S. Yu, D.J. Lim, and X.-X. Gu (2011) Intranasal immunization of the combined lipooligosaccharide conjugates protects mice from the challenges with three serotypes of Moraxella catarrhalis. PLoS ONE:e29553.
- Choi, B.Y., H.-M. Kim, T. Ito, K.-Y. Lee, X. Li, K. Monahan, Y. Wen, E. Wilson, K. Kurima, T.L. Saunders, R.S. Petralia, P. Wangemann, T.B. Friedman, and A.J. Griffith (2011) Mouse model of enlarged vestibular aqueducts defines temporal requirement of Slc26a4 expression for hearing acquisition. J. Clin. Inv. 121:4516-4525.
- Wang, P.Y., R.S. Petralia, Y.-X. Wang, R.J. Wenthold, and S.D. Brenowitz (2011) Functional NMDA receptors at axonal growth cones of young hippocampal neurons. J. Neurosci. 31:9289-9297.
- Ren, D., S. Yu, S. Gao, D. Peng, R.S. Petralia, A. Muszynski, R.W. Carlson, J.B. Robbins, C.-M. Tsai, D.J. Lim, X.-X. Gu (2011) Mutant Lipooligosaccharide-based conjugate vaccine demonstrates a broad-spectrum effectiveness against Moraxella catarrhalis. Vaccine 29:4210-4217.
- Zheng, C.-Y., R.S. Petralia, Y.-X. Wang, and B. Kachar (2011) Fluorescence recovery after photobleaching (FRAP) of fluorescence tagged proteins in dendritic spines of cultured hippocampal neurons. Journal of Visualized Experiments (DOI: 10.3791/2568; www.jove.com).
- Zheng, C.-Y., Y.-X. Wang, B. Kachar, and R.S. Petralia (2011) Differential localization of SAP102 and PSD-95 is revealed in spines using super-resolution light microscopy. Communicative & Integrative Biology 4:104-105.
- Zheng, C.-Y., R.S. Petralia, Y.-X. Wang, B. Kachar, and R.J. Wenthold (2010) SAP102 is a highly mobile MAGUK in spines. J. Neurosci. 30:4757-4766.
- Petralia, R.S., Y.X. Wang, F. Hua, Z. Yi, A. Zhou, L. Ge, F.A. Stephenson, and R.J. Wenthold (2010) Organization of NMDA receptors at extrasynaptic locations. Neuroscience 167:68-87 (corrigendum in Neuroscience 176:472, 2011).
- Suh, Y.H., A. Terashima, R.S. Petralia, R.J. Wenthold, J.T.R. Isaac, K.W. Roche, and P.A. Roche (2010) A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking. Nat. Neurosci. 13:338-343.
Selected research papers from outside collaborations, published since 2010
- Mitchell, N.*, R.S. Petralia*, P.J. Yao, D.G. Currier, Y.-X. Wang, A. Kim, M.P. Mattson (2012) Sonic hedgehog regulates presynaptic terminal size, ultrastructure and function in hippocampal neurons (*co-1st authors). J. Cell Sci. (in press).
- Kim, J., T. Zhao, R.S. Petralia, Y. Yu, H. Peng, E. Myers, and J.C. Magee (2012) mGRASP enables mapping mammalian synaptic connectivity with light microscopy. Nat. Meth. 9:96-102.
- Wu, J., R.S. Petralia, H. Kurushima, H. Patel, M. Jung, L. Volk, S. Chowdhury, J.D. Shepherd, M. Dehoff, Y. Li, D. Kuhl, R.L. Huganir, D.L. Price, R. Scannevin, J.C. Troncoso, P.C. Wong, and P.F. Worley (2011) Arc/Arg3.1 regulates an endosomal pathway essential for activity-dependent b-amyloid generation. Cell 147:615-628.
- Sun, W., J.K. Maffie, L. Lin, R.S. Petralia, B. Rudy, and D.A. Hoffman (2011) DPP6 establishes the A-type K+ current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons. Neuron 71:1102-1115.
- Bangash, M.A., J.M. Park, T. Melnikova, D. Wang, S.K. Jeon, D. Lee, S. Syeda, J. Kim, M. Kouser, J. Schwartz, Y. Cui, X. Zhao, H. Speed, S. Kee, J.C. Tu, R.S. Petralia, D.J. Linden, C. Powell, A. Savonenko, B. Xiao, P.F. Worley (2011) Enhanced Polyubiquitination of Shank3 and NMDA receptor in a mouse model of Autism. Cell 145:758-772.