Advanced Imaging Core
Ronald S. (Ron) Petralia, Ph.D.
Ya-Xian Wang, M.D.
Ronald S. (Ron) Petralia, Ph.D., Staff Scientist
Porter Neuroscience Research Center
35A Convent Drive 1E614
Bethesda, MD 20892-3729 for U.S. Postal Service
Bethesda, MD 20814 for other carriers (FedEx, UPS, etc.)
Telephone: (301) 496-3804 (office)
Dr. Petralia received a B.S. from University of Massachusetts Amherst in 1975, and a Ph.D. from Texas A&M University in 1979, and has been a practicing electron microscopist for 35 years. He practiced light and electron microscopic immunocytochemistry at the NIDCD, in the laboratory of Dr. Robert J. Wenthold (deceased) from 1991-2009, prior to establishing the Advanced Imaging Core.
Ya-Xian Wang, M.D., Biologist
Phone: (301) 496-3805
Dr. Wang earned an M.D. from Harbin Medical University, P.R. China in 1977, then was Doctor-in-Charge in Ophthalmology/Eye Surgery in Heilongjiang Provincial Hospital in Harbin until she came to the United States. She worked in the laboratory of Dr. Robert J. Wenthold from 1993-2009 on electron microscopy immunocytochemistry and primary neuron cell culture.
The Advanced Imaging Core (AIC) offers investigators specialized centralized core laboratory support services. The primary goal of the AIC is to support the research interests and ongoing projects of various NIDCD laboratories within the Division of Intramural Research (DIR). The AIC provides the resources necessary to assist in the proper performance of a variety of specialized experiments and in the interpretation of obtained data. The AIC provides specific expertise, new technologies, and resources to enhance the research efforts of all NIDCD investigators. The team specializes in transmission electron microscope (TEM) studies of immunocytochemistry and ultrastructure, but can also do scanning electron microscope (SEM) and confocal and other light microscope (LM) analyses (the latter for correlative LM/EM studies).
Cell biological studies, including physiology, genetics, immunocytochemistry, etc., always consider what happens at the molecular level. Transmission electron microscopy allows scientists to come closer than ever to studying cell composition at the molecular level. The AIC’s TEM specialists offer training and related assistance to principal investigators at the NIDCD, and contribute to collaborative studies with scientists at the NIDCD, NIH, and beyond.
Tissue preparation with freeze-substitution
NIDCD research papers published since 2010
Fujikawa, T., R.S. Petralia, T.S. Fitzgerald, Y.-X. Wang, B. Millis, J.A. Morgado-Díaz, K. Kitamura, and B. Kachar (2014) Localization of kainate receptors in inner and outer hair cell synapses. Hearing Res.
- He, S., Y.-X. Wang, R.S. Petralia, and S. Brenowitz (2014) Cholinergic modulation of large-conductance calcium-activated potassium channels regulates synaptic strength and spine calcium in cartwheel cells of the dorsal cochlear nucleus. J. Neurosci. 34:5261-5272.
- Brusco, J., S. Merlo, E.T. Ikeda, R.S. Petralia, B. Kachar, A. A. Rasia-Filho, and J.E. Moreira (2014) Inhibitory and multisynaptic spines, and hemispherical synaptic specialization in the posterodorsal medial amygdala of male and female rats. J. Comp. Neurol. 522:2075-2088.
- Szarama, K.B., N. Gavara, R.S. Petralia, R.S. Chadwick, and M.W. Kelley (2013) Thyroid hormone increases fibroblast growth factor receptor expression and disrupts cell mechanics in the developing organ of Corti. BMC Devel. Biol. (online).
- Szarama, K.B., R. Stepanyan, R.S. Petralia, N. Gavara, G.I. Frolenkov, M.W. Kelley, and R.S. Chadwick (2012) Fibroblast growth factor receptor 3 regulates microtubule formation and cell surface mechanical properties in the developing organ of Corti. Bioarchitecture 2:214-219.
- Standley, S.H., R.S. Petralia, M. Gravell, R. Hamilton, Y.-X. Wang, M. Schubert, and R.J. Wenthold (2012) Trafficking of NMDAR2A/B receptor subunit distal cytoplasmic tails from endoplasmic reticulum to the synapse. PLoS ONE 7(6):e39585.
- Szarama, K.B., N. Gavara, R.S. Petralia, M.W. Kelley, and R.S. Chadwick (2012) Cytoskeletal changes in actin and microtubules underlie the developing surface mechanical properties of sensory and supporting cells in the mouse cochlea. Development 139:2187-2197.
- Seabold, G.K., P.Y. Wang, R.S. Petralia, K. Chang, A. Zhou, M.I. McDermott, Y.-X. Wang, S.L. Milgram, and R.J. Wenthold (2012) Dileucine and PDZ binding motifs mediate synaptic adhesion-like molecule 1 (SALM1) trafficking in hippocampal neurons. J. Biol. Chem. 287:4470-4484.
- Ren, D., H. Xie, W. Zhang, F. Hassan, R.S. Petralia, S. Yu, D.J. Lim, and X.-X. Gu (2011) Intranasal immunization of the combined lipooligosaccharide conjugates protects mice from the challenges with three serotypes of Moraxella catarrhalis. PLoS ONE:e29553.
- Choi, B.Y., H.-M. Kim, T. Ito, K.-Y. Lee, X. Li, K. Monahan, Y. Wen, E. Wilson, K. Kurima, T.L. Saunders, R.S. Petralia, P. Wangemann, T.B. Friedman, and A.J. Griffith (2011) Mouse model of enlarged vestibular aqueducts defines temporal requirement of Slc26a4 expression for hearing acquisition. J. Clin. Inv. 121:4516-4525.
- Wang, P.Y., R.S. Petralia, Y.-X. Wang, R.J. Wenthold, and S.D. Brenowitz (2011) Functional NMDA receptors at axonal growth cones of young hippocampal neurons. J. Neurosci. 31:9289-9297.
- Ren, D., S. Yu, S. Gao, D. Peng, R.S. Petralia, A. Muszynski, R.W. Carlson, J.B. Robbins, C.-M. Tsai, D.J. Lim, X.-X. Gu (2011) Mutant Lipooligosaccharide-based conjugate vaccine demonstrates a broad-spectrum effectiveness against Moraxella catarrhalis. Vaccine 29:4210-4217.
- Zheng, C.-Y., R.S. Petralia, Y.-X. Wang, and B. Kachar (2011) Fluorescence recovery after photobleaching (FRAP) of fluorescence tagged proteins in dendritic spines of cultured hippocampal neurons. Journal of Visualized Experiments (DOI: 10.3791/2568; www.jove.com).
- Zheng, C.-Y., Y.-X. Wang, B. Kachar, and R.S. Petralia (2011) Differential localization of SAP102 and PSD-95 is revealed in spines using super-resolution light microscopy. Communicative & Integrative Biology 4:104-105.
- Zheng, C.-Y., R.S. Petralia, Y.-X. Wang, B. Kachar, and R.J. Wenthold (2010) SAP102 is a highly mobile MAGUK in spines. J. Neurosci. 30:4757-4766.
- Petralia, R.S., Y.X. Wang, F. Hua, Z. Yi, A. Zhou, L. Ge, F.A. Stephenson, and R.J. Wenthold (2010) Organization of NMDA receptors at extrasynaptic locations. Neuroscience 167:68-87 (corrigendum in Neuroscience 176:472, 2011).
- Suh, Y.H., A. Terashima, R.S. Petralia, R.J. Wenthold, J.T.R. Isaac, K.W. Roche, and P.A. Roche (2010) A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking. Nat. Neurosci. 13:338-343.
Selected research papers from outside collaborations, published since 2010
- Wyeth, M.S., K.A. Pelkey, R.S. Petralia, M.W. Salter, R.R. McInnes, and C.J. McBain (2014) Neto auxillary protein interactions regular kainate & NMDA receptor subunit localization at mossy fiber-CA3 pyramidal cell synapses. J. Neurosci. (in press).
- Hou, H., L. Sun,, B.A. Siddoway, R.S. Petralia, H. Yang, H. Gu, A.C. Nairn, and H. Xia (2013) Synaptic NMDA receptor stimulation activates PP1 by inhibiting its phosphorylation by Cdk5. J. Cell Biol. doi: 10.1083/jcb201303035. Featured in “In This Issue” in J. Cell Biol. and EurekAlert! from Rockefeller University Press.
- Petralia, R.S., Y.-X. Wang, F.E. Indig, I. Bushlin, F. Wu, M.P. Mattson, and P.J. Yao (2013) Regulation of synaptic vesicle size and density by AP180 and CALM. NeuroMol. Med. 15:49-60.
- Petralia, R.S., C.M. Schwartz, Y.-X. Wang, E.M. Kawamoto, M.P. Mattson, and P.J. Yao (2013) Sonic hedgehog promotes autophagy in hippocampal neurons. Biol. Open 2:499-504.
- Siddoway, B., J. Altimimi, H. Hou, R.S. Petralia, B. Xu, D. Stellwagen, and H. Xia (2013) An essential role for inhibitor-2 regulation of protein phosphatase-1 in synaptic scaling. J. Neurosci. 33:11206-11.
- Hu, J.-H., L. Yang, P.J. Kammermeier, C.G. Moore, P.R. Brakeman, J. Tu, S. Yu, R.S. Petralia, Z. Li, P.-W. Zhang, J.M. Park, X. Dong, B. Xiao, and P.F. Worley (2012) Preso1 dynamically regulates group 1 metabotropic glutamate receptors. Nat. Neurosci. 15:836-844 (also featured in “News and Views” in issue, pp. 805-807).
- Yang, Y., X. Shu, D. Liu, Y. Shang, Y. Wu, L. Pei, X. Xu, Q. Tian, J. Zhang, K. Qian, Y.-X. Wang, R.S. Petralia, W. Tu, L.-Q. Zhu, J.-Z. Wang, and Y. Lu (2012) EPAC null mutation impairs learning and social interactions via aberrant regulation of miR-124 and Zif268 translation. Neuron 73:774-788.
- Mitchell, N.*, R.S. Petralia*, P.J. Yao, D.G. Currier, Y.-X. Wang, A. Kim, and M.P. Mattson (2012) Sonic hedgehog regulates presynaptic terminal size, ultrastructure and function in hippocampal neurons. J. Cell Sci. 125:4207-4213 (*co-1st authors).
- Kim, J., T. Zhao, R.S. Petralia, Y. Yu, H. Peng, E. Myers, and J.C. Magee (2012) mGRASP enables mapping mammalian synaptic connectivity with light microscopy. Nat. Meth. 9:96-102.
- Wu, J., R.S. Petralia, H. Kurushima, H. Patel, M. Jung, L. Volk, S. Chowdhury, J.D. Shepherd, M. Dehoff, Y. Li, D. Kuhl, R.L. Huganir, D.L. Price, R. Scannevin, J.C. Troncoso, P.C. Wong, and P.F. Worley (2011) Arc/Arg3.1 regulates an endosomal pathway essential for activity-dependent b-amyloid generation. Cell 147:615-628.
- Sun, W., J.K. Maffie, L. Lin, R.S. Petralia, B. Rudy, and D.A. Hoffman (2011) DPP6 establishes the A-type K+ current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons. Neuron 71:1102-1115.